Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

摘要: Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds various tissue engineering applications. However, one limitation electrospun from need to cross-link with glutaraldehyde stability, which has been postulated lead many complications in vivo including graft failure. In this study, we determined characteristics hybrid composed collagen and elastin, as well gelatin, polymer poly(epsilon-caprolactone) (PCL), so avoid chemical cross-linking. Fiber size increased proportionally increasing protein concentrations, whereas pore decreased. Electrospun gelatin/PCL showed higher tensile strength when compared collagen/elastin/PCL constructs. To determine effects on cell attachment migration, both were seeded adipose-derived stem cells. Scanning electron microscopy nuclei staining cell-seeded demonstrated complete surfaces scaffolds, although migration into scaffold was predominantly seen hybrid. The combination create structures resulted favorable mechanical biological properties.