Microbial lipopeptides stimulate dendritic cell maturation via Toll-like receptor 2.

摘要: The ability of dendritic cells (DC) to initiate immune responses in naive T is dependent upon a maturation process that allows the develop their potent Ag-presenting capacity. Although immature DC can be derived vitro by treatment peripheral blood monocytes with GM-CSF and IL-4, additional signals such as those provided TNF-alpha, CD40 ligand, or LPS are required for complete maximum APC function. Because we recently found microbial lipoproteins activate through Toll-like receptor (TLR) 2, also investigated whether drive maturation. Immature were cultured without monitored expression cell surface markers indicative Stimulation lipopeptides increased CD83, MHC class II, CD80, CD86, CD54, CD58, decreased CD32 endocytic activity; these lipopeptide-matured displayed enhanced stimulatory capacity MLR, measured proliferation IFN-gamma secretion. lipid moiety lipopeptide was essential induction Preincubation maturing an anti-TLR2 blocking Ab before addition blocked phenotypic functional changes associated These results demonstrate stimulate via TLR2, providing mechanism which products bacteria participate initiation response.