Differences in CART expression and cell cycle behavior discriminate sympathetic neuroblast from chromaffin cell lineages in mouse sympathoadrenal cells.

摘要: Adrenal medullary chromaffin cells and peripheral sympathetic neurons originate from a common sympathoadrenal (SA) progenitor cell. The timing phenotypic changes that mark this lineage diversification are not fully understood. present study investigated the expression patterns of markers, cell cycle dynamics, in adrenal medulla neighboring suprarenal ganglion embryonic mice. noradrenergic marker, tyrosine hydroxylase (TH), was detected both presumptive cells, but with significantly stronger immunostaining former. There intense cocaine amphetamine-regulated transcript (CART) peptide most neuroblasts, whereas very few showed detectable CART immunostaining. This segregation appeared as early E12.5, before anatomical two types. Cell dynamics were also examined. Initially, 88% Sox10 positive (+) neural crest progenitors proliferating at E10.5. Many SA withdrew E11.5 they started to express TH. Whereas 70% neuroblasts (TH+/CART+ cells) back only around 20% (CART negative) E12.5 subsequent days. Thus, neuroblast lineages differences proliferative behavior their earliest appearance. We conclude intensity TH permit discrimination developing exhibit lower activity relative neuroblasts. © 2015 Wiley Periodicals, Inc. Develop Neurobiol,