Glucocorticoid receptor binding to a specific DNA sequence is required for hormone-dependent repression of pro-opiomelanocortin gene transcription.
作者： J Drouin , M A Trifiro , R K Plante , M Nemer , P Eriksson
关键词:
摘要: Glucocorticoids rapidly and specifically inhibit transcription of the pro-opiomelanocortin (POMC) gene in anterior pituitary, thus offering a model for studying negative control mammals. We have defined an element within rat POMC 5'-flanking region that is required glucocorticoid inhibition POMC-expressing pituitary tumor cells (AtT-20). This contains vitro binding site purified receptor. Site-directed mutagenesis revealed receptor to this located at position base pair -63 essential repression transcription. Although related well-defined response (GRE) found glucocorticoid-inducible genes, DNA sequence (nGRE) differs significantly from GRE consensus; divergence may result different receptor-DNA interactions account least part opposite transcriptional properties these elements. Hormone-dependent be due over positive regulatory promoter. Thus, mutually exclusive two DNA-binding proteins overlapping sequences.
sci-hub.se 参考文章(66)
T. Perlmann, O. Wrange, Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome. The EMBO Journal. ,vol. 7, pp. 3073- 3079 ,(1988) , 10.1002/J.1460-2075.1988.TB03172.X
F R Weiner, M J Czaja, D M Jefferson, M A Giambrone, R Tur-Kaspa, L M Reid, M A Zern, The effects of dexamethasone on in vitro collagen gene expression. Journal of Biological Chemistry. ,vol. 262, pp. 6955- 6958 ,(1987) , 10.1016/S0021-9258(18)48184-7
Y P Hwung, L H Wang, S Y Tsai, M J Tsai, Differential binding of the chicken ovalbumin upstream promoter (COUP) transcription factor to two different promoters. Journal of Biological Chemistry. ,vol. 263, pp. 13470- 13474 ,(1988) , 10.1016/S0021-9258(18)37729-9
H. Richard-Foy, G. L. Hager, Sequence-specific positioning of nucleosomes over the steroid-inducible MMTV promoter. The EMBO Journal. ,vol. 6, pp. 2321- 2328 ,(1987) , 10.1002/J.1460-2075.1987.TB02507.X
O Wrange, S Okret, M Radojćić, J Carlstedt-Duke, J A Gustafsson, Characterization of the purified activated glucocorticoid receptor from rat liver cytosol. Journal of Biological Chemistry. ,vol. 259, pp. 4534- 4541 ,(1984) , 10.1016/S0021-9258(17)43079-1
J H Eberwine, J L Roberts, Glucocorticoid regulation of pro-opiomelanocortin gene transcription in the rat pituitary. Journal of Biological Chemistry. ,vol. 259, pp. 2166- 2170 ,(1984) , 10.1016/S0021-9258(17)43332-1
SM Frisch, HE Ruley, Transcription from the stromelysin promoter is induced by interleukin-1 and repressed by dexamethasone. Journal of Biological Chemistry. ,vol. 262, pp. 16300- 16304 ,(1987) , 10.1016/S0021-9258(18)49254-X
S A Camper, Y A Yao, F M Rottman, Hormonal regulation of the bovine prolactin promoter in rat pituitary tumor cells. Journal of Biological Chemistry. ,vol. 260, pp. 12246- 12251 ,(1985) , 10.1016/S0021-9258(17)39017-8
O Wrange, J Carlstedt-Duke, J A Gustafsson, Stoichiometric analysis of the specific interaction of the glucocorticoid receptor with DNA. Journal of Biological Chemistry. ,vol. 261, pp. 11770- 11778 ,(1986) , 10.1016/S0021-9258(18)67310-7
Jacques Drouin, Howard M. Goodman, Most of the coding region of rat ACTH beta--LPH precursor gene lacks intervening sequences. Nature. ,vol. 288, pp. 610- 613 ,(1980) , 10.1038/288610A0