Immunohistochemistry for the prion protein: Comparison of different monoclonal antibodies in human prion disease subtypes

摘要: Demonstration of the abnormal form prion protein (PrP) in brain confirms diagnosis human disease (PrD). Using immunohistochemistry, we have compared ten monoclonal antibodies PrD subtypes including sporadic and variant Creutzfeldt-Jakob (CJD), fatal familial insomnia, Alzheimer's (AD), control brains. CJD subgroups were determined using Western blot analysis for protease-resistant PrP type combination with sequencing to determine genotype at methionine/valine polymorphism codon 129 gene. None labeled given exclusively, but intensity immunoreactivity varied among morphologically distinct types deposit. Fine granular or synaptic deposits stained weakly not all against N-terminus PrP, visible one case only 12F10 SAF54. Coarser plaque immunolabeled antibodies. The immunostaining patterns appear characteristic subgroups. Labeling certain neurons cases irrespective disease, staining periphery and/or throughout senile plaques AD patients also noted. Antibodies such as 6H4 failed give this labeling are therefore less likely recognise non-pathological material immunohistochemistry.