Isolation and characterization of proteoheparan sulfate from plasma membranes of an ascites hepatoma, AH 66.

摘要: A proteoglycan was isolated from plasma membranes prepared AH 66 cells by the following procedure. The were according to method devised Funakoshi and Yamashina (1976) J. Biochem. 80, 1185-1193), then made lipid-free. lipid-free solubilized with 5 mM sodium phosphate buffer, pH 7.0, containing 0.5% dodecyl sulfate (SDS), solution fractionated on a Sepharose CL 6B column. eluted near void volume fraction further purified repeated precipitation cetylpyridinium chloride (CPC). homogeneous electrophoresis cellulose acetate strip identified as proteoheparan sulfate. preparation contained 10.6% protein, its amino acid composition being characterized high contents of glutamic acid, aspartic proline, glycine, threonine, serine.