Immobilization of invertase in germania matrix and a study of its enzymatic activity

摘要: Invertase from baker yeast was entrapped within germania nanospheres. The enzyme incorporated simultaneously during the precipitation of using peptide sequence T-G-H-Q-S-P-G-A-Y-A-A-H that is known to catalyze formation germania. efficiency immobilization and its activity under different conditions, operation storage stability were studied. efficiently found be stable retained over a longer period time compared free in solution. effect temperature pH on showed remained active range 15–65 °C. Optimum immobilized invertase at 60 In addition, broader (4.5–6.5). Thus nanospheres can immobilize enzymes remain temperatures. Such techniques protect harsh environments required chemical synthesis functionalization methods. ability trap biomolecules such as gives rise new class materials with variety applications biosensors bioactive glasses.