HxlR, a member of the DUF24 protein family, is a DNA-binding protein that acts as a positive regulator of the formaldehyde-inducible hxlAB operon in Bacillus subtilis.

摘要: The HxlR protein from Bacillus subtilis belongs to the DUF24 family (InterPro No. IPR002577) of unknown function. hxlR gene that encodes this is located upstream hxlAB operon. This operon two key enzymes in ribulose monophosphate pathway are involved formaldehyde fixation, 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. Expression induced by presence formaldehyde. Recombinant prepared Escherichia coli showed specific binding a region DNA Using gel-retardation DNase I footprinting assays, we identified 25 bp regions for within DNA. Surface plasmon resonance analyses suggested dimers sequentially bound Finally, demonstrated each was necessary formaldehyde-induced expression B. subtilis. Thus, have shown DNA-binding