Deep Quantitative Proteomics Reveals Extensive Metabolic Reprogramming and Cancer-Like Changes of Ectopic Endometriotic Stromal Cells.

摘要: Endometriosis is a prevalent health condition in women of reproductive age characterized by ectopic growth endometrial-like tissue the extrauterine environment. Thorough understanding molecular mechanisms underlying disease still incomplete. We dissected eutopic and endometrial primary stromal cell proteomes to depth nearly 6900 proteins using quantitative mass spectrometry with spike-in SILAC standard. Acquired data revealed metabolic reprogramming cells extensive upregulation glycolysis downregulation oxidative respiration, widespread phenotype known as Warburg effect previously described many cancers. These changes metabolism are additionally accompanied attenuated aerobic respiration measured live-cell oximetry altered mRNA levels respective enzyme complexes. Our results highlight other endometriotic cel...