A Novel Impedimetric Based Screening System for Label-Free Detection of Tau Hyperphosphorylation in Human Cells

摘要: Alzheimer’s disease (AD) is the most common neurodegenerative that affects more than 4 million people in Europe. AD a member of group neurological disorders collectively referred as tauopathies, which are char acterized by aggregation hyperphosphorylated tau lead ing to neuronal cytotoxicity and cognitive impairments. Given complex pathological mechanism, drug development pro grams for rely heavily on animal models, making research very expensive time-consuming. In this context lack fast, label-free high throughput/high content screening capable cell-based assays bottleneck today’s active pharmaceutical ingredient development. To improve identification, optimization validation during high-cost Hit-to-Lead cycle drugs we report first time an vitro model, where consequences induced hyperphosphorylation can be detected quantitatively microelectrode array based impedance spec troscopy. Our findings provide novel real-time recording technique testing efficiency kinase inhibitors or other structures directed differentiated SH-SY5Y cells.