Remodeling of organelle-bound actin is required for yeast vacuole fusion

摘要: Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles absence cytosol or cytoskeleton, regulates last compartment mixing stage homotypic vacuole fusion. The Cdc42p GTPase is known be required for show proteins Cdc42p-regulated actin remodeling cascade (Cdc42p → Cla4p Las17p/Vrp1p Arp2/3 complex actin) are enriched on isolated vacuoles. Vacuole fusion dramatically altered by perturbation vacuole-bound either mutation ACT1 gene, addition specific ligands such as latrunculin B jasplakinolide, antibody regulatory Las17p (yeast Wiskott-Aldrich syndrome protein) Arp2/3, deletion genes. On docked vacuoles, at “vertex ring” membrane microdomain where occurs and terminal steps leading This role may extend other systems.