Untersuchungen zum Nachweis von Erwinia carotovora subsp. atroseptica (van Hall) Dye in Kartoffelknollen mit Hilfe des ELISA-Testverfahrens
作者： Rum Zielke , Johannes Richter , Ilona Kalinina
DOI: 10.1016/S0232-4393(86)80072-5
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摘要: Summary The usefulness of the microliter-ELISA (enzyme-linked immunosorbent assay) for detection soft rot pathogen Erwinia carotovora subsp. atroseptica (van Hall) Dye is demonstrated. For this purpose, suspensions causal agent and potato tubers latently infected were tested. In both cases, 1 × 10 4 cells/ml was low critical concentration which could be detected. This agrees well with dates published by other authors. Presumption application ELISA to identify E. a useful antiserum — beside its specificity allows high dilution Immunoglobuline conjugate. By technique exact about latent infestation under test can obtained.
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