Slow release of soluble TNF receptors by monocytes in vitro.

摘要: In this study, we investigated the release of soluble(s) TNF-R by PBMC in vitro. T cell activation mAb anti-CD3, as well with phorbol esters (PMA), enhanced both sTNF-R55 and sTNF-R75 PBMC. contrast to shedding neutrophils upon activation, sTNF-R proved be a relatively slow process, reaching plateau after 2 days culture. Monocytes appeared main source released sTNF-R, whereas purified cells induced only minor compared whole population. To unravel mechanism, number cytokines were added during 2-day culture cells. IL-10 levels similar kinetics anti-CD3 PMA, other tested did not affect PBMC, pure lymphocytes, or monocytes, either activated not. Conversely, inhibitors period study effect endogenously produced on release. anti-IL-10 IL-1ra partly release, The results obtained vitro may extend our insight mechanism via which are vivo inflammatory reactions.