Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus.

摘要: Toll-like receptor 2 (TLR2) in mammals is a member of the ancient family receptors that predominantly recognizes conserved components Gram-positive bacteria. In present study, tlr2 gene and its 5'-flanking sequence were cloned from turbot, Scophthalmus maximus, responsive expressions to various immunostimulants subsequently studied vivo. The turbot (sm)tlr2 spans over 9.0 kb with structure 12 exon-11 intron encodes 816 amino acids. deduced protein shows highest identity (76.1%) Japanese flounder Tlr2 possesses signal peptide sequence, leucine-rich repeat (LRR) domain composed 19 LRR motifs, transmembrane region Toll/interleukin-1 (TIR) domain. Phylogenetic analysis grouped it other neoteleostei Tlr2as. A number transcription factor binding sites known be important for basal transcriptional activity TLR3 response TLR2 lipopolysaccharide (LPS) signalling predicted smtlr2. Quantitative real-time PCR (qPCR) demonstrated constitutive expression smtlr2 mRNA all twelve examined tissues higher levels lymphomyeloid-rich liver. Further, was up-regulated following stimulation LPS, peptidoglycan (PGN) or polyinosinic: polycytidylic acid [poly(I:C)] gills, head kidney, spleen muscle. Finally, three immunostimulants, two-wave induced observed kidney 7-day time course strongest inducibility kidney. These findings suggest possible role Smtlr2 immune responses infections broad range pathogens include Gram-negative bacteria RNA virus.