Calibration for Isotope Dilution Mass Spectrometry—Description of an Alternative to the Bracketing Procedure

摘要: A calibration method for isotope dilution mass spectrometry is presented that fully accounts non-linearity of functions, caused by the interference analyte on m/z used measurement internal standard and vice versa. In this way, it also possible to use incompletely labelled molecules or analogues with a increment only 1 u dilution, condition certain restrictions are respected. addition, proposed less time consuming than bracketing. The works computer-stored full curve single point measured daily. constructed from experimentally determined spectral overlap between unlabelled at values chosen measurement. Measurement results samples unknown concentration calculated basis linear relationship their ion abundance ratio daily point, but corrected factor derived theoretical function. All calculations performed commonly available spreadsheet software. An application determining serum uric acid candidate gas chromatographic/ spectrometric reference method.