Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release

摘要: Peptides have great potential in the treatment of various diseases because their high specificity and safety. However, application has been limited by lack appropriate delivery techniques. In this study, we demonstrated intracellular peptides using viral nanoparticles (VNPs) bacteriophage P22 through covalent loading two different with synergistic cytotoxic effects controllable release triggered Cathepsin B, a highly over-expressed protease many tumors. Fluorescence imaging localization VNPs evidenced that system could be transported as designed. Cell Counting Kit-8 (CCK-8) assay flow cytometry analysis revealed on MDA-MB-231 tumor cells. These results confirmed feasibility genetically fusing cargo to capsid protein cleavable thereafter. This study may instructive for design drug systems multifunctional theranostic devices based VNPs.