Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway

摘要: Background It is of importance to minimize ischemia reperfusion (I/R) injury during liver operations. Reducing the inflammatory reaction an effective way achieve this goal. Notably, adiponectin (APN) was found have anti-inflammatory activity in heart and renal I/R injury. Herein, we investigated role APN injury. Methods Wistar rats were randomized four groups: (1) sham group; (2) control (3) I/R+APN (4) I/R+APN+AMPK inhibitor group. Liver blood samples collected 6h 24h after reperfusion. function histopathologic changes assessed. Macrophage neutrophil infiltration detected by immunohistochemistry staining, while pro-inflammatory cytokines chemokines released measured using ELISA RT-PCR, respectively. Apoptosis analyzed TUNEL staining caspase-3 expression liver. Downstream molecules Western blotting. Results Circulatory down-regulated I/R. When exogenous treatment administered I/R, alanine transaminase (ALT) aspartate aminotransferase (AST) decreased, less hepatocyte necrosis observed. Less cell cytokines/chemokines release also observed group when compared with reduced apoptosis, evidenced positive cells reperfused Finally, AMPK/eNOS pathway be activated APN, administration AMPK reversed beneficial effects APN. Conclusion APN can protect from reducing response a process that likely involves pathway. The current study provides potential pharmacologic target for