A novel lipopeptide produced by a Pacific Ocean deep‐sea bacterium, Rhodococcus sp. TW53

摘要: Aims:  Our goal was to find a novel, biosurfactant-producing bacterium from Pacific Ocean deep-sea sediments. Methods and Results:  An oil-degrading TW53 obtained sediment, identified through 16S rDNA analysis as belonging the genus Rhodococcus. It lowered surface tension of its culture 34·4 mN m−1. Thin layer chromatography (TLC) showed that crude biosurfactants were composed lipopeptides free fatty acids (FA). The purified with column then hydrolysed 6 mol l−1 HCl. Gas chromatography-mass spectrometry hydrolyte in hydrophobic fraction contained five kinds FA chain lengths C14–C19, C16H32O2 major component making up 59·18% total. However, 3-hydroxyl not found, although it is usually found lipopeptides. Silica gel TLC revealed hydrophilic amino acids; consistently, ESI-Q-TOF-MS confirmed composition results provided their sequence tentatively Ala-Ile-Asp-Met-Pro. Furthermore, yield CMC (critical micelle concentrations) examined. product reduced water 30·7 mN m−1 value 23·7 mg l−1. These suggest Rhodococcus sp. produces novel lipopeptide we have named rhodofactin. Conclusion:  isolate first reported lipopeptide-producing this genus. had chemical compositions. Significance Impact Study: Rhodococcus has potential exploration new could be used bioremediation marine oil pollution.