Pectate lyases of Erwinia chrysanthemi: Pel E-like polypeptides and pelE homologous sequences in strains isolated from different plants☆☆☆
作者： K.K. Thurn , F. Barras , Y. Kegoya-Yoshino , A.K. Chatterjee
DOI: 10.1016/0885-5765(87)90055-5
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摘要: Pel E, one of the four major pectate lyases produced by Erwinia chrysanthemi (Echr) strain EC16, was purified to homogeneity and found have an apparent molecular weight 47 500 a pI 10. Antibodies against this preparation inhibited E activity, but did not affect A, B or C activities. Immunotitration revealed that accounted for fraction total extracellular activity ranging from 40–60% in culture potato tuber tissue. Isoelectric focusing Pels various Echr strains indicated while profiles isolated hosts were different, same host very similar. A significant proportion (ranging 39 74%) these anti-Pel antibodies. DNA hybridization under stringent conditions presence pelE homologous sequences genomes E. strains. We conclude E-like enzyme occurs all examined.
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