Rat forebrain perfusion in vivo by 1 artery like the isolated kidney model: a robust recovery model permitting ischemia without anesthesia to compare multiple brain injury states.

摘要: BACKGROUND Rat brain perfusion models are critical to basic research, but they can be imprecise and/or not durable for extended outcome studies. OBJECTIVE To demonstrate a rat model that provides simplified reliable circuit, reduces variables during experiment and recovery, therefore permits more precise, reliable, context-independent research data. METHODS forebrain was reduced surgically by 1 internal carotid artery without injury the animal. The next day, fully awake studied ischemia painlessly yet in absence of anesthesia or other interventions might bias alter biochemistry event. This rigorously validated with isotope cerebral blood studies histology at 72 hours after ischemia. first application this compare injuries global total, penumbra, shock single experimental context. RESULTS is accurate, remarkably durable. severest vessel occlusion ever demonstrated recovery model. It also confirms that, conditions otherwise identical, penumbra less injurious than total CONCLUSION Although meticulous construction, creates simply reliably Pulsinelli 4-vessel inspired it, inherent advantages an isolated organ system, which known tissue volume perfused predetermined rate. robust long-term recovery.