Molecular cloning of a gene encoding a seed-specific protein with nutritionally balanced amino acid composition from Amaranthus.

摘要: An albumin with a well-balanced amino acid composition and high levels of the essential acids was purified to homogeneity from mature seeds Amaranthus hypochondriacus. The this protein is comparable World Health Organization recommended values for highly nutritional protein. 35-kDa monomer four isoforms that can be separated by chromatofocusing. Antibodies raised against one isoforms, AmA1, cross-reacted other three isoforms. Affinity-purified AmA1 antibodies were used isolate cDNA clones developing-seed expression library. six immunopositive recombinants obtained found related. largest clone (1.2 kilobases) has single major open reading frame corresponding 304-amino polypeptide. confirmed hybrid-selected translation immunoprecipitation. size immunoprecipitated product identical Analysis RNA in developing showed synthesized during early embryogenesis, reaching maximum midmaturation. No detected 1-day-old seedlings although delayed breakdown on germination. Expression gene seed-specific, as no or plant tissues.