Measuring Colocalization by Dual Color Single Molecule Imaging: Thresholds, Error Rates, and Sensitivity
作者： Verena Ruprecht , Julian Weghuber , Stefan Wieser , Gerhard J. Schütz
DOI: 10.1016/B978-0-12-381266-7.00002-X
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摘要: Abstract Dual color single molecule tracking is a state-of-the-art fluorescence microscopy technique to study molecular associations. Featuring high spatial and temporal resolution, the method allows for catching associations with extremely reliability. In this review, we describe robust data analysis approaches identify characterize from dual images show how colocalization can be statistically discriminated accidental colocalizations. We discriminate case of static where colocalizations are revealed individual images, dynamic tracked in time.
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