作者: Tiago Pereira Arantes , Welber Daniel Zanetti Lopes , Roberta Machado Ferreira , Juliana S. Pinto Pieroni , Vanessa M.R. Pinto
DOI: 10.1016/J.EXPPARA.2009.07.003
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摘要: Abstract Ten male dogs were distributed into three experimental groups for infection with Toxoplasma gondii : GI – inoculated 2.0 × 10 5 P strais oocysts, GII infected 1.0 × 10 6 RH strain tachyzoites, and GIII four controls dogs. Several clinical parameters evaluated. IFAT was performed to detect anti- T. antibodies. Presence of the parasite in semen evaluated by PCR bioassay techniques. Tissue parasitism examined using bioassays immunohistochemistry testicle epididymis fragments collected after orchiectomy. In samples from these two groups, presence verified PCR. detected tissues (testicle fragments) all six experimentally The -positive seminal used artificial insemination (AI) female free toxoplasmic infection. Seven days AI, presented serologic conversion (IFAT). Fetal reabsorption occurred dogs, while others sustained full-term gestation. cysts brains offspring. These results suggest that can be sexually transmitted domestic