High performance capillary electrophoresis for determination of the enantiomers of 2-arylpropionic acid derivatives in human serum. Pharmacokinetic studies of ketoprofen enantiomers following administration of standard and sustained release tablets.

摘要: A stereospecific capillary zone electrophoresis (CZE) method for determination of the enantiomers some 2-arylpropionic acid derivatives (2-APA, profens) in human serum has been developed. Racemic-ibuprofen (rac-IBP), racemic-flurbiprofen (rac-FBP), racemic-ketoprofen (rac-KTP) and (+)-S-naproxen ((+)-S-NPX--an internal standard) were chosen these studies. The 2-APA extracted from acidified samples using methylene chloride separated a fused silica capillary. was filled with background electrolyte, which consisted 0.05 M heptakis 2,3,6-tri-O-methyl-beta-cyclodextrin (TMbetaCD) (chiral selector) 0.02 triethanolamine-phosphate buffer pH 5.0. Separation resolution enantiomer mixture obtained one analytical run. calculated electrophoretic parameters analytes as follows: mobility, micorep(-)-R = -0.75 x 10(-4) to -0.30 cm2/Vs; micorep(+)-S -0.83 -(-0.38) cm2/Vs electroosmotic microEOF 2.35 cm2/Vs, migration times, tmigr R 12.55 - 16.07 min; s 13.08 16.9 min, factors, RS 1.88 3.70 chiral selectivity, alpha 1.16 1.34. developed validated. calibration curves linear range 0.5-50.0 microg/ml FBP or KTP 1.0-50.0 IBP concentrations. Recovery about 90%. At limit quantification (LOQ) precision accuracy within 15%. validated successfully applied pharmacokinetic bioavailability studies on humans after administration standard sustained release tablets rac-KTP. Significant differences both formulations observed studied not bioequivalent.