Treatment with a Zinc Metalloprotease Purified from Bothrops moojeni Snake Venom (BmooMP-Alpha-I) Reduces the Inflammation in an Experimental Model of Dextran Sulfate Sodium-Induced Colitis.

摘要: It has been described that the metalloprotease BmooMP-alpha-I purified from Bothrops moojeni snake venom is able to hydrolyze TNF molecule. However, this observation based mainly on in vitro investigation, addition molecular modeling and docking approaches. Considering there no vivo study demonstrate biological effects of enzyme, major aim present work was investigate whether any anti-inflammatory efficacy by setting up a murine experimental design colitis induced dextran sulfate sodium (DSS). For purpose, C57BL/6 mice were divided into six groups, as follows: (i) animals without intestinal inflammation, (ii) inflammation treated with (50 μg/animal/day), (iii) 3% DSS, (iv) DSS enzyme at 50, 25, or 12.5 μg/animal/day dosages intraperitoneal route. Clinical signs observed daily for calculating morbidity scores, cytokine measurements, histological features. We different doses presented remarkable improvement signs, confirmed significant increase intestine length comparison group. Also, difference between groups vehicle, colon these slightly lower than group healthy animals, induction inflammation. The quantification supernatants tissue homogenates showed reduction 38% IFN-gamma levels, when 50 μg compared receiving only. A 39% levels also all treatment BmooMP-alpha-I, 35% amount IL-12p40. Histological examinations revealed preserved architecture goblet cells reduced ulcer area, mice, which typical inflammatory changes architecture, such ulceration, crypt dilation, loss cell depletion, accompanied infiltration. In conclusion, our results suggest clinical scores findings related model could be attributed ability modulate production locally inflamed intestine. These highlight potential role effectiveness therapeutic alternative type immunopathological condition.