Putative identification of the usnic acid biosynthetic gene cluster by de novo whole-genome sequencing of a lichen-forming fungus.
作者： Mona Abdel-Hameed , Robert L. Bertrand , Michele D. Piercey-Normore , John L. Sorensen
DOI: 10.1016/J.FUNBIO.2015.10.009
关键词:
摘要: To identify the biosynthetic gene cluster responsible for biosynthesis of polyketide usnic acid we carried out de novo genome sequencing fungal partner Cladonia uncialis. This was followed by comprehensive in silico annotation synthase (PKS) genes. The requires a non-reducing PKS possessing carbon methylation (CMeT) domain, terminal Claisen cyclase (CLC) and an accompanying oxidative enzyme that dimerizes methylphloracetophenone to acid. Of 32 candidate genes identified mycobiont genome, only one as consistent with these requirements. contains two encoding cytochrome p450, which have been respectively named (MPAS) oxidase (MPAO). Both mpas mpao were demonstrated be transcriptionally active reverse transcriptase-PCR mRNA lichen sample observed HPLC produce Phylogenetic analysis bioinformatically ketosynthase (KS) CLC domains MPAS grouped within unique clade could used phylogenetic probe other
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