Discovery and Cross-Amplification of Microsatellite Polymorphisms in Asterinid Sea Stars

摘要: Variation in tandem repeats of two- to six-base nucleotide motifs (microsatellites) can be used obtain inexpensive and highly informative multi-locus data on population genetics.We developed tested a large set cross-amplifiable sea star (Asterinidae) microsatellite markers from mixed pool genomic DNA eight species. We describe cloned sequences, primers, PCR conditions, characterize population-level variation for some species markers. A few clones containing microsatellites showed considerable similarity sequences (including genes known function) other stars urchins (from the Strongylocentrotus purpuratus complete genome). The pooled method was an efficient way sample many species: we 2–10 each species, most could cross-amplified 1–7 At 12 loci two found 1–10 alleles per microsatellite, with broad range inbreeding coefficients. Measure...