An HPTLC method for quantification of cholesteryl esters from human plasma and rat liver microsomes

摘要: Cholesteryl oleate present as a neutral lipid in low-density lipoprotein has been speculated to be biomarker for atherosclerosis. Methods which are at hand the quantification of cholesteryl either costly or entail use radioactive compounds. Charring TLC plates used identify esters long time but never applied biological matrices. Here, we report novel method based on planar chromatography analysis products acyl CoA–cholesterol acyltransferase (ACAT) assay, viz. esters. Using silica gel 60 F254 stationary phase, compounds were spotted plate and run using solvent system comprising n-hexane–diethyl ether–glacial acetic acid (90:10:1, v/v/v). The developed by dipping anisaldehyde–sulfuric reagent scanned 546 nm quantification. shows good linear relationship concentration range 100–500 ng/band with correlation coefficient (r) value 0.9996. was validated accuracy, precision robustness. Percentage recovery found 96.88–103.01% intra- inter-day yielding <2% relative standard deviation nominal concentrations analysis. limits detection 6.45 19.54 ng, respectively. robustness making deliberate changes mobile phase composition, volume temperature analysis, deviations peak areas these intentional 1.07, 1.02 1.30 estimation plasma samples from patients diagnosed hypercholesterolemia. No interferences matrices assay. proposed could immense potential marker ACAT activity, screening inhibitors drug discovery process prognosis Copyright © 2013 John Wiley & Sons, Ltd.