Detection of human papillomaviruses in tissue specimens.

摘要: During the past decade, molecular methods based on detection of viral DNA have become a key tool for human papillomaviruses (HPVs) in tissue. The can be divided into two groups: those which tissue destruction is unavoidable HPV DNA, and performed way that allows morphology preservation. Polymerase chain reaction currently most sensitive method an excellent research tool. However, because frequent contamination problems lack standardization, it not readily applicable to diagnostic laboratories. recent improvements situ hybridization made possible this appropriate routine HPVs At present, however, use at least independent indispensable accurate determination HPVs.