Cultural Estimation of Yeasts on Seaweeds

摘要: Five per cent suspensions of freshly harvested seaweeds were used as an inoculum to develop a selective medium for epiphytic yeasts. Conditions satisfactory yeast growth and visualization red colonies on membrane filters obtained by supplementing basal glucose-Trypticase-yeast extract-agar at pH 7.0 with 100 mg each chloramphenicol 2,3,5-triphenyl tetrazolium chloride liter. Maximal counts triturating the algae in prechilled (4 C) seawater blender 2 5 min. Inhibitory phenolic materials released from phaeophytes during this process removed modified Cholodny filtration. A preliminary survey indicated that yeasts all nine species maximal populations occurred chlorophytes rhodophytes especially periods warmer water.