Biological and molecular basis of human breast cancer

摘要: Human breast cancer remains the most common malignancy in American women. The ultimate cure of this disease relies on a better understanding mechanisms underlying initiation and progression disease. neoplastic transformation HBEC vitro represents successful model for obtaining knowledge molecular biological alterations that may contribute to tumorigenic mechanisms. We have presented here current chemically transformed following aspects: 1. Factors affecting such as genetic predisposition differentiation status prior immortalization; 2. New targets studying mechanism cell immortalization telomerase activity differential expression cycle dependent genes well others recently isolated through cloning H-ferritin, calcium binding protein; 3. Epigenetic transformation; 4. association microsatellite instability specific loci chromosomes 11, 13, 16 with 5. application microcell mediated chromosome transfer technique an approach testing functional role whose dysregulation or loss function transformation. Further efforts system will be directed determine roles identified changes mapping/cloning tumor suppressor senescence those reside 11 17.