Temporal speckle-averaging of optical coherence tomography volumes for in-vivo cellular resolution neuronal and vascular retinal imaging

作者: Pengfei Zhang , Eric B. Miller , Suman K. Manna , Ratheesh K. Meleppat , Edward N. Pugh

DOI: 10.1117/1.NPH.6.4.041105

关键词:

摘要: It has been recently demonstrated that structures corresponding to the cell bodies of highly transparent cells in retinal ganglion layer could be visualized noninvasively living human eye by optical coherence tomography (OCT) via temporal averaging. Inspired this development, we explored application volumetric averaging mice, which are important models for studying diseases and therapeutic interventions. A general framework speckle-averaging (TSA) OCT angiography (OCTA) is presented applied mouse data. Based on image analysis, eyes mice under anesthesia exhibit only minor motions, lateral displacements a few micrometers rotations fraction 1 deg. Moreover, due reduced movements anesthesia, there negligible amount motion artifacts within volumes need corrected achieve volume coregistration. In addition, relatively good quality ocular media allows cellular-resolution imaging without adaptive optics (AO), greatly simplifying experimental system, making proposed feasible large studies. The TSA OCTA results provide rich information about new previously not with non-AO-OCT. mechanism relies improving signal-to-noise ratio as well efficient suppression speckle contrast decorrelation patterns, enabling full utilization high resolution offered OCTA.

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