Susceptibility of insulin to proteolysis in rat lung homogenate and its protection from proteolysis by various protease inhibitors

摘要: The objective of this study was to determine the stability insulin in rat lung homogenate and confirm effectiveness various protease inhibitors for delivery from lung. compared with that small intestine. Insulin rapidly degraded similarly degradation intestinal homogenate. effects on were studied Protease such as Na-glycocholate (Na-GC ; 10mм), aprotinin (10mg/ml), soybean trypsin inhibitor (STI 10mg/ml) bacitracin (20mм) effectively reduced rank order decreasing hydrolysis bacitracin>aprotinin>STI>Na-GC. In addition, a slight correlation observed between situ pulmonary absorption its vitro presence inhibitors. These findings suggest coadministration would be useful improving