Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch ( Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing
作者： Konstantin V. Krutovsky , Iraida N. Tretyakova , Nataliay V. Oreshkova , Maria E. Pak , Olga V. Kvitko
DOI: 10.1007/S11627-014-9619-Z
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摘要: The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping 11 microsatellite loci showed high levels genomic instability frequency mutation the obtained megagametophyte-derived callus cultures. All cultures contained new mutations one or more loci.
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