Increased phosphorylation on residue S795 of the retinoblastoma protein in esophageal adenocarcinoma.

摘要: Due to its increasing incidence and relatively poor prognosis, esophageal adenocarcinoma (EAC) is becoming a significant health problem. Elucidating the mechanisms underlying EAC development of great importance improve upon current conventional treatment strategies. Insight into phosphorylation has proven be useful for diagnostic molecular strategies in cancer. A pathway largely dependent on frequently deregulated cancer cell cycle regulating p16-retinoblastoma (Rb) pathway. We investigated kinase activity, specifically within p16-Rb pathway, EAC. high-throughput peptide tyrosine array containing short peptides representing 100 proteins with known sites, was used assess activity Also, specific changes protein Rb upstream regulator P16 were validated through immunoblotting normal cells tissues. Phosphorylation higher tissues as compared squamous majority significantly phosphorylated found involved structure maintenance immunity. Validation biopsy specimens lines showed hyper associated aberrant expression The (S795) residue tissue (Wilcoxon paired rank test, p=0.004). Investigation may indicate targets intervention give more insight