Genetic analysis of selected strains of eastern oyster (Crassostrea virginica Gmelin) using AFLP and microsatellite markers.
作者： Ziniu Yu , Ximing Guo
DOI: 10.1007/S10126-004-3600-5
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摘要: Amplified fragment length polymorphisms (AFLPs) and microsatellite markers were used to examine genetic variation divergence in 4 selected strains (DBH, NEH, FMF, CTS) 1 wild population (DBW) of the eastern oyster Crassostrea virginica Gmelin. Eighty-six AFLP (from 3 primer pairs) 5 loci for analysis 30 oysters from each populations. Microsatellite considerably more variable than AFLPs. The observed heterozygosity ranged 0.560 0.640 across populations microsatellites, 0.186 0.207 Both F st φPT data statistics revealed significant between all pairs There was no reduction strains; however, number alleles per locus lower population. Two subjected long-term selection disease resistance shared frequency shifts at a few loci, which deserve further determine if they are linked disease-resistance genes.
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