Novel Approach to the Detection and Quantification of Phenolic Compounds in Olive Oil Based on 31P Nuclear Magnetic Resonance Spectroscopy

摘要: 31P NMR spectroscopy has been employed to detect and quantify phenolic compounds in the polar fraction of virgin olive oil. This novel analytical method is based on derivatization hydroxyl carboxyl groups with 2-chloro-4,4,5,5-tetramethyldioxaphospholane identification phosphitylated basis chemical shifts. Quantification a large number oil can be accomplished by integration appropriate signals spectrum use cyclohexanol as internal standard. Finally, validity this technique for quantitative measurements was thoroughly examined. Keywords: Olive oil; compounds;