Increased isoprene production using mevalonate kinase and isoprene synthase

摘要: The invention features methods for producing isoprene from cultured cells having increased expression levels and/or activity of a mevalonate kinase polypeptide and an synthase polypeptide. also provides reduced accumulation intermediates (such as mevalonate, isopentenyl diphosphate, 3,3-dimethylallyl geranyl or farnesyl diphosphate) in the biosynthesis isoprenoids that may otherwise cause undesirable amounts growth inhibition, toxicity, cell death. resulting compositions have yields purity isoprene.