Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney.
作者： Eisei Sohara , Tatemitsu Rai , Sung-Sen Yang , Akihito Ohta , Shotaro Naito
DOI: 10.1371/JOURNAL.PONE.0024277
关键词:
摘要: The NaCl cotransporter (NCC) is essential for sodium reabsorption at the distal convoluted tubules (DCT), and its phosphorylation increases transport activity apical membrane localization. Although insulin has been reported to increase in kidney, linkage between NCC not yet investigated. This study examined whether regulates phosphorylation. In cultured mpkDCT cells, increased of STE20/SPS1-related proline-alanine-rich kinase (SPAK) a dose-dependent manner. insulin-induced was suppressed WNK4 SPAK knockdown cells. addition, Ly294002, PI3K inhibitor, decreased effect on phosphorylation, indicating that induces through Moreover, acute administration mice oxidative stress-responsive kinase-1 (OSR1), kidney. Time-course experiments cells suggested upstream this mechanism, which confirmed by lack knockout mice. hypomorphic did OSR1, suggesting also involved mechanism vivo. present results demonstrated potent regulator are insulin.
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