BRK tyrosine kinase expression in a high proportion of human breast carcinomas.

摘要: BRK is a recently described non receptor protein tyrosine kinase whose mRNA was found to be expressed in human breast tumours and cancer cell lines. Expression of fibroblasts mammary epithelial cells has been shown enhance their ability grow anchorage independently, expressing acquire potentiated mitogenic response epidermal growth factor. In order investigate further the expression cancers, we have isolated monoclonal antibodies specifically recognising protein. Whereas low or undetectable normal tissue benign lesions, approximately two-thirds appreciable levels, 27% over by fivefold more (up 43×). This pattern mirrored comparison lines derived either from carcinomas. constant throughout cycle, did not vary with proliferation rate. A consideration this data, conjunction BRK's demonstrated deregulate cells, supports hypothesis that high proportion carcinomas functionally important factor evolution.