The Structure of Microbial Communities in Soil and the Lasting Impact of Cultivation.

摘要: The structure of microbial communities was examined as a function community composition and the relative abundance specific groups to examine effects that plant land-use history have on in soil. sites sampled were part Long Term Ecological Research (LTER) project agricultural ecology at W.K. Kellogg Biological Station Michigan State University (Hickory Corners, MI) included both active abandoned fields well nearby had never been cultivated. Microbial assessed by extracting total RNA from soil samples using 16S rRNA-targeted oligonucleotide probes quantify rRNA alpha, beta, gamma Proteobacteria, Actinobacteria (Gram positive bacteria with high mol % G+C genome), Bacteria, Eukarya. In addition, characterized examining fluorescently tagged terminal restriction fragment length polymorphisms (T-RFLP) PCR amplified rDNA. observed be remarkably similar among plots shared long-term management despite differences land maintained recent years. contrast, differed significantly between cultivated those having cultivation.