A partial methylation profile for a CpG site is stably maintained in mammalian tissues and cultured cell lines.
作者： M S Turker , K Swisshelm , A C Smith , G M Martin
DOI: 10.1016/S0021-9258(18)80110-7
关键词:
摘要: We wished to determine if a partial methylation profile for specific CpG site was stably maintained in both mammalian tissues and cultured cell lines. To accomplish this, we identified with located upstream of the mouse adenine phosphoribosyltransferase promoter region. This found be methylated at level approximately 25% brain, kidney, lung, skeletal muscle tissues, close 50% liver, 0% testis. These tissue-specific profiles were not altered during aging. A this also observed five teratocarcinoma stem lines one additional line. profile, however, upon cellular differentiation, hemizygosity, loss activity some conclude that can vivo vitro mechanism exists its maintenance. The functional significance remains determined.
elsevier.com
sci-hub.st 参考文章(27)
R. J. S. Reis, S. Goldstein, Variability of DNA methylation patterns during serial passage of human diploid fibroblasts. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 3949- 3953 ,(1982) , 10.1073/PNAS.79.13.3949
M. K. Dush, J. M. Sikela, S. A. Khan, J. A. Tischfield, P. J. Stambrook, Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 2731- 2735 ,(1985) , 10.1073/PNAS.82.9.2731
Michael W. McBurney, Brenda J. Rogers, Isolation of male embryonal carcinoma cells and their chromosome replication patterns Developmental Biology. ,vol. 89, pp. 503- 508 ,(1982) , 10.1016/0012-1606(82)90338-4
Howard Cedar, DNA methylation and gene activity Cell. ,vol. 53, pp. 3- 4 ,(1988) , 10.1016/0092-8674(88)90479-5
Judith L. Swain, Timothy A. Stewart, Philip Leder, Parental legacy determines methylation and expression of an autosomal transgene: A molecular mechanism for parental imprinting Cell. ,vol. 50, pp. 719- 727 ,(1987) , 10.1016/0092-8674(87)90330-8
R. Stein, N. Sciaky-Gallili, A. Razin, H. Cedar, Pattern of methylation of two genes coding for housekeeping functions. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 2422- 2426 ,(1983) , 10.1073/PNAS.80.9.2422
Yosef Gruenbaum, Howard Cedar, Aharon Razin, Restriction enzyme digestion of hemimethylated DNA Nucleic Acids Research. ,vol. 9, pp. 2509- 2515 ,(1981) , 10.1093/NAR/9.11.2509
Ram P. Singhal, Laura L. Mays-Hoopes, Gunther L. Eichhorn, DNA methylation in aging of mice. Mechanisms of Ageing and Development. ,vol. 41, pp. 199- 210 ,(1987) , 10.1016/0047-6374(87)90040-6
Robert J. Shmookler Reis, Samuel Goldstein, Interclonal variation in methylation patterns for expressed and non-expressed genes. Nucleic Acids Research. ,vol. 10, pp. 4293- 4304 ,(1982) , 10.1093/NAR/10.14.4293
P. H. Yen, P. Patel, A. C. Chinault, T. Mohandas, L. J. Shapiro, Differential methylation of hypoxanthine phosphoribosyltransferase genes on active and inactive human X chromosomes. Proceedings of the National Academy of Sciences. ,vol. 81, pp. 1759- 1763 ,(1984) , 10.1073/PNAS.81.6.1759