Abstract B32: Myeloid-derived suppressor cell depletion augments antitumor activity in ovarian cancer

摘要: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid that increased in tumors and create an immunosuppressive environment by inhibiting the T-cell function. In addition, MDSCs promote angiogenesis, tumor invasion, metastasis. Increased MDSC accumulation epithelial ovarian cancer (EOC) has been associated with poor prognosis. Our study investigated whether depletion will influence EOC progression enhance therapeutic response programmed death-1 (PD1) immunotherapy. The intraperitoneal ID8 syngeneic mouse cell model B6 mice was used for study. tumor-bearing were treated once week either anti-Gr1 specific monoclonal antibody (Ly6G/Ly6C mAb) targets depletes MDSCs, or its isotype IgG2b mAb as control (100µg/dose/mouse). Mice sacrificed at day 60 burden evaluation. Quantification various immune their effector cytokines blood, spleen, bone marrow, ascites, performed fluorescence-activated sorter (FACS) using surface intracellular markers, ELISA, immunohistochemistry (IHC). preclinical trial anti-GR1 anti-PD1 Ab combination also carried out. ID8-bearing exhibited significantly higher levels (CD11bGr1+) (p Citation Format: Sharif Sakr, Sajad Dar, Shailendra Giri, Adnan Munkarah, Ramandeep Rattan. augments antitumor activity cancer. [abstract]. In: Proceedings AACR Conference: Addressing Critical Questions Ovarian Cancer Research Treatment; Oct 1-4, 2017; Pittsburgh, PA. Philadelphia (PA): AACR; Clin Res 2018;24(15_Suppl):Abstract nr B32.