Morphological, Molecular Identification and Distribution of Trypanosome-Transmitting Dipterans from Cattle Settlements in Southwest Nigeria.

摘要: Glossina spp. (Glossinidae), Tabanus (Tabanidae), Ancala Atylotus (Tabanidae) and Stomoxys (Muscidae) are important transmitting vectors of African animal trypanosomosis in sub-Saharan Africa. There is paucity information on the distribution identification these flies cattle settlements southwest Nigeria. The patterns, genetic variations diversities dipteran Nigeria were described identified using morphological molecular analysis 28S rDNA gene. Of 13,895 examined morphologically between April 2016 March 2017, tabanids [Tabanus (0.34%), (0.03%), (0.01%), Haematopota (0.014%) Chrysops (0.11%)]. Two stomoxyine species identified; niger Macquart (45.30%) calcitrans Linnaeus (17.29%) two namely; p. gambiense Vanderplank, 1911 (0.46%) tachinoides Westwood (0.51%) identified. identities further confirmed a BLAST search their nucleotide sequences. median-joining network gene sequences indicated that fly genetically distinct. apparent density all trapped was highest at mean temperature 26–28 ℃, humidity > 80% rainfall 150–220 mm/month. observed to increase as vegetation increased decreased areas with relatively high human population (> 100/km2). indices suggest nuclear DNA fragments may provide more ecology flies. Characterising assessing impact essential monitoring AAT spread.