Identification and characterization of differentially expressed genes in the resistance reaction in taro infected with Phytophthora colocasiae

摘要: Leaf blight disease caused by Phytophthora colocasiae represents a major constraint to the growth and yield of taro (Colocasia esculenta L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated endogenous molecule such as salicylic acid, jasmonic ethylene. Activation defenses is associated with changes in expression large number genes. To gain better understanding responses, virulent race P. was used inoculate cultivar UL-56 (compatible) its nearly isogenic line Muktakeshi (incompatible). We have employed suppressive subtractive hybridization (SSH), cDNA libraries, Northern blot analysis, high throughput DNA sequencing, bioinformatics identify defense-related genes induced infection. Two putative resistance transcription factor were identified among upregulated sequences. The several candidate including lipid transfer proteins (LTPs), other pathogenesis-related evaluated following 8–48 h appearance symptom compatible incompatible interactions. Results confirmed higher overall these (resistant) compared (susceptible). This study constitutes first attempt characterize differential transcriptome host–pathogen interactions from different genotypes. All generated ESTs been submitted GenBank for further functional studies.