Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.

摘要: Oligodendrocyte precursor cells (NG2 glia) are uniformly distributed proliferative in the mammalian central nervous system and generate myelinating oligodendrocytes throughout life. A subpopulation of OPCs neocortex arises from progenitor embryonic ganglionic eminences that also produce inhibitory neurons. The neuronal fate some is sealed before birth as they become committed to oligodendrocyte lineage, marked by sustained expression transcription factor Olig2, which represses interneuron Dlx2. Here we show misexpression Dlx2 alone postnatal mouse caused them switch their GABAergic neurons within 2 days downregulating Olig2 upregulating a network neuron transcripts. After two weeks, OPC-derived generated trains action potentials formed clusters synaptic proteins. Our study revealed developmental molecular logic can be applied promote reprogramming OPCs.