Comparative mapping of genes from human chromosome 12 by genetic linkage mapping in cattle

摘要: Loci from human chromosome 12 were mapped in cattle to compare the gene order between species. Polymorphisms detected six loci that had been with high precision humans. Four of these loci, LALBA, SLC2A3, SYT1, and TPI1, bovine 5, one, PLA2G1B, 17. The sixth locus, SLC2A3L, due a fragment produced by SLC2A3 primers, maps telomeric region BTA18. differences when are added others already cattle, show evidence significant rearrangement requiring several evolutionary events. There is also 5 interspersal material conserved on 22 into 12, consistent ZOOFISH analyses. This analysis indicates larger block near centromere long arm smaller telomere as part short 12. level variation amplified DNA was approximately 1 variant per 464 nucleotides haploid using single-strand conformation polymorphism analysis. corresponds individual 1, 961 DNA. confirms lower genetic variability compared humans but potential for millions single nucleotide polymorphisms cattle.