Dynamics extracted from fixed cells reveal feedback linking cell growth to cell cycle

摘要: A mathematical method, known as ergodic rate analysis, has been developed and used to study the rates of molecular events from single time measurements large populations fixed cells; this new method is able overcome some previous limitations with regards studying cell-size control. How cell size maintained a fundamental question in biology. In study, Marc Kirschner colleagues describe analytical called analysis (ERA), which measures dynamics cellular processes based on single-cell steady-state populations. They use monitor how proliferating cells constrain variation size, calculate growth relation their position cycle. The results suggest that just before S phase, there sharp transition dependence acts limit variation. Biologists have long concerned about what constrains but progress field slow stymied by experimental limitations1. Here we uses accurately infer events, including growth. ERA exploits fact number particular state related average transit through state2. With it possible full trajectories any feature can be labelled cells, for example levels phosphoproteins or total mass. Using find evidence size-discriminatory process at G1/S decrease cell-to-cell