CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age

摘要: The hematopoietic stem cell (HSC) compartment is heterogeneous, yet our understanding of the identities different HSC subtypes limited. Here we show that platelet integrin CD41 (αIIb), currently thought to only transiently mark fetal HSCs, expressed on an adult subtype accumulates with age. CD41+ HSCs were largely quiescent and exhibited myeloerythroid megakaryocyte gene priming, governed by Gata1, whereas CD41- more proliferative lymphoid priming. When isolated without use blocking antibodies, possessed long-term repopulation capacity serial transplantations showed a marked myeloid bias compared which yielded lymphoid-biased progeny. CD41-knockout (KO) mice displayed multilineage defects coupled decreased quiescence survival suggesting functionally relevant for maintenance homeostasis. Transplantation experiments indicated CD41-KO-associated are transplantable, HSC-derived and, in part, mediated through loss mass leading decreases exposure important released cytokines, such as transforming growth factor β1. In summary, data provide novel marker identify myeloid-biased becomes prevalent age highlights dogma regulation their