Extracellular Vesicles Derived from Primary Adipose Stromal Cells Induce Elastin and Collagen Deposition by Smooth Muscle Cells within 3D Fibrin Gel Culture.

摘要: Macromolecular components of the vascular extracellular matrix (ECM), particularly elastic fibers and collagen fibers, are critical for proper physiological function arteries. When unique biomechanical combination these is disrupted, or in ultimate extreme where completely lost, arterial disease can emerge. Bioengineers realms tissue engineering regenerative medicine must therefore ideally consider how to create engineered grafts containing right balance develop treatments situations such as an aneurysm have been lost. Previous work has demonstrated that primary cells responsible ECM production during development, smooth muscle (SMCs), be induced make new when exposed secreted factors from adipose-derived stromal cells. To further dissect this signal transmitted, study, were partitioned into vesicle (EV)-rich EV-depleted fractions well unseparated controls. EVs validated using electron microscopy, dynamic light scattering, protein quantification before testing biological effects on SMCs. In 2D culture, promoted SMC proliferation migration. After 30 days 3D fibrin construct transcription microfibril gene FBN1 deposition insoluble elastin collagen. Uniaxial properties strand constructs no different after EV treatment versus summary, it apparent some positive elastogenesis mediated by EVs, indicating a potential use therapy restore disease.